Differential quantification of CYP2D6 gene copy number by four different quantitative real-time PCR assays
نویسندگان
چکیده
منابع مشابه
Determination of Cytochrome P450 2D6 (CYP2D6) Gene Copy Number by Real-Time Quantitative PCR
Gene dosage by real-time quantitative PCR has proved to be accurate for measuring gene copy number. The aim of this study was to apply this approach to the CYP2D6 gene to allow for rapid identification of poor and ultrarapid metabolizers (0, 1, or more than 2 gene copy number). Using the 2(-Delta Delta Ct) calculation method and a duplex reaction, the number of CYP2D6 gene copies was determined...
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متن کاملDetermination of CYP2D6 gene copy number by pyrosequencing.
BACKGROUND Identification of CYP2D6 alleles *5 (deletion of the whole CYP2D6 gene) and *2xN (gene duplication) is very important because they are associated with decreased or increased metabolism of many drugs. The most commonly used method for analysis of these alleles is, however, considered to be laborious and unreliable. METHODS We developed a method to determine the copy number of the CY...
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ژورنال
عنوان ژورنال: Pharmacogenetics and Genomics
سال: 2010
ISSN: 1744-6872
DOI: 10.1097/fpc.0b013e32833a1083